Scaled-Down Nuclease P1 for Scaled-Up DNA Digestion
نویسندگان
چکیده
منابع مشابه
Scaled-down nuclease P1 for scaled-up DNA digestion.
Motivated by an interest in detecting DNA adducts in their nucleotide form by mass spectrometry (1), we sought efficient, low-cost conditions for enzymatic digestion of a relatively large amount of DNA (≥ 1 mg). Of the enzymes available for such digestion, nuclease P1, which forms deoxynucleoside-5′-monophosphates, seemed to be a good initial choice because it is widely used for digesting DNA, ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2003
ISSN: 0736-6205,1940-9818
DOI: 10.2144/03345bm01